It is actually necessary for individuals to use a quality dissolved oxygen test kit. This is for the sake of determining what the health of an aquatic ecosystem is. This should also help in evaluating productivity and pollution in areas like lakes, streams, and rivers. Here are the important steps that people should take when they want to use this item.
The preparation of the BOD bottle is the first step that people will have to consider. It should be obvious but this BOD bottle will have to be filled up with sample H2O. When filling up this bottle, be sure that there are no air bubbles mixing into the sample. Just tilt the sample-filled bottle on either side to let these bubbles escape.
Prepare the manganese sulfate as well. Add this to the sample using a calibrated pipette. The said pipette should be inserted into the H2O's surface, squeeze it slowly after that. When doing this, one has to make sure that no air bubbles are being introduced in this sample to avoid altering the result.
It is also a given to pay close attention to the alkali-iodide-azide reagent. One will have to use this as this will be added to one's sample. Since the person is going to add this alkali-iodide-azide reagent into one's sample, one has to make use of the pipette as well. There should be less difficulties in adding this reagent into one's sample.
Carefully put the stopper into the bottle, making sure that there are no air introduced inside. One should then turn the BOD bottle upside down. Do this step several times just to mix its contents. Do not forget to check whether the sample has bubbles or not. If there are air bubbles, then that means one needs to discard the said sample and then start over.
Sulfuric acid will have to be added to this sample too. The person will have to utilize the calibrated pipette as well when it comes to the addition of the sulfuric acid into this sample. This time around, instead of dipping this pipette below the surface, one just needs to tip the pipette about the surface. Stopper the bottle again and turn it over, mixing the content.
Prepare an Erlenmeyer flask beforehand too. The Erlenmeyer flask is where the person will need to put a 201 ml of one's sample into. For the tester to obtain the correct measurement of the sample, the person should get a graduated cylinder and use it. Get a calibrated pipette afterward and then add the sodium thiosulfate to change the color to pale straw.
A starch solution should then be added to the sample. Two ml of this should do. After adding this to the solution, the sample should change into a blue color. After that, add more sodium thiosulfate until it changes into a clear color.
One should then record the total amount of the added sodium thiosulfate that one has added into the solution. This is the best way to calculate the O content of this sample. When using the dissolved oxygen test kit, every sodium thiosulfate one has added means that the sample contains one milligram per liter of dissolved oxygen.
The preparation of the BOD bottle is the first step that people will have to consider. It should be obvious but this BOD bottle will have to be filled up with sample H2O. When filling up this bottle, be sure that there are no air bubbles mixing into the sample. Just tilt the sample-filled bottle on either side to let these bubbles escape.
Prepare the manganese sulfate as well. Add this to the sample using a calibrated pipette. The said pipette should be inserted into the H2O's surface, squeeze it slowly after that. When doing this, one has to make sure that no air bubbles are being introduced in this sample to avoid altering the result.
It is also a given to pay close attention to the alkali-iodide-azide reagent. One will have to use this as this will be added to one's sample. Since the person is going to add this alkali-iodide-azide reagent into one's sample, one has to make use of the pipette as well. There should be less difficulties in adding this reagent into one's sample.
Carefully put the stopper into the bottle, making sure that there are no air introduced inside. One should then turn the BOD bottle upside down. Do this step several times just to mix its contents. Do not forget to check whether the sample has bubbles or not. If there are air bubbles, then that means one needs to discard the said sample and then start over.
Sulfuric acid will have to be added to this sample too. The person will have to utilize the calibrated pipette as well when it comes to the addition of the sulfuric acid into this sample. This time around, instead of dipping this pipette below the surface, one just needs to tip the pipette about the surface. Stopper the bottle again and turn it over, mixing the content.
Prepare an Erlenmeyer flask beforehand too. The Erlenmeyer flask is where the person will need to put a 201 ml of one's sample into. For the tester to obtain the correct measurement of the sample, the person should get a graduated cylinder and use it. Get a calibrated pipette afterward and then add the sodium thiosulfate to change the color to pale straw.
A starch solution should then be added to the sample. Two ml of this should do. After adding this to the solution, the sample should change into a blue color. After that, add more sodium thiosulfate until it changes into a clear color.
One should then record the total amount of the added sodium thiosulfate that one has added into the solution. This is the best way to calculate the O content of this sample. When using the dissolved oxygen test kit, every sodium thiosulfate one has added means that the sample contains one milligram per liter of dissolved oxygen.
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