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Selasa, 11 Agustus 2015

The Process Of Equine DNA Testing

By Eula Clarke


DNA is the blueprint of all living things. It contains information that dictates how they breathe, eat, reproduce and look like. DNA technology has revolutionized how we do things. It enables one to determine parentage, predict the appearance of a phenotype or even a disease present in the parent population to the offspring. This technology is not only available for humans but also for animals such as horses. Equine DNA testing is used on horses.

Horses are tested for a variety of reasons. For instance, one may want to know the parents of a foal for breeding purposes. Scientific determination of pedigree information is imperative for accuracy purposes. Traditional methods such as observation, record keeping, and manual identification are not only inadequate but also inaccurate.

During breeding, a breeder identifies animals with better performance, endurance or other characteristics that are desirable. It is only DNA technology that can ensure this is done accurately. It also helps determine whether a horse is at risk of developing a certain genetic disease and also to choose some phenotypic characteristics through constructing a genetic profile.

DNA can be tested in various genetic laboratories around the world. A simple search online reveals many companies. They have a set of sample collection procedures and guidelines that one may follow in order to extract the sample to be used for the test. After collection, the samples are sent to the laboratories through post service from anywhere around the world.

The cost of the test depends on the type of test to be carried out. Also, the results can be sent to you, or you can view them online as most of these laboratories have websites. Company representatives also call to explain the results and what they mean for the horse. The results are usually in a chart form and are easily explained to the horse owner.

The collection of samples for testing is not a difficult process. Most tests will require hair from the mane or the tail. Other samples may include cells collected from inside the horses cheek by use of a soft cheek brush. Some other tests may require blood as sample. In such cases, 3ml whole blood is collected using a syringe and stored in an EDTA Vacutainer and labeled. After labeling, it may then be sent to the lab through regular mail.

When these samples arrive at the laboratory, DNA is extracted. It is extracted from the hair follicle cells in the hair sample and from the nucleus in the cells of this cheek or the blood. After extraction, twelve specific gene markers are tested to produce a profile or genotype of the equine. Note that each of the markers bears a pair of alleles, and each pair is from each parent.

DNA technology is now part and parcel of everyone of us. It has made our lives better on Earth. Now we can use it on animals during breeding to ensure that we yield high performing horses that are not prone to disease. Also, it is now possible to know the parentage of a horse before breeding and be 100% sure.




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